Glen o Glenda

Lugosi Béla

Scientist

Edward D. Wood Jr.

Glen / Glenda (as Daniel Davis)

Lyle Talbot

Inspector Warren

Timothy Farrell

Dr. Alton / Narrator

Dolores Fuller

Barbara

'Tommy' Haynes

Alan / Anne

Charlie Crafts

Johnny (as Charles Crafts)

Conrad Brooks

Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)

Henry Bederski

Man with Hat and Receding Hairline (uncredited)

Carol Daugherty

Woman in Nightmare (uncredited)

'Captain' DeZita

The Devil / Glen's Father (uncredited)

Helen Miles

(uncredited)

Shirley Speril

Miss Stevens (uncredited)

Amzie Strickland

Minor Role (uncredited)

Harry Thomas

Man in Nightmare (uncredited)

William C. Thompson

Judge (uncredited)

'Mr. Walter' Hadjwiecyz

Patrick / Patricia (uncredited)

George Weiss

Man at Transvestite's Suicide (uncredited)

Evelyn Wood

Sheila, Glen's Sister (uncredited)

Edward D. Wood Jr.

Story, Director, Screenplay

William C. Thompson

Director of Photography

George Weiss

Producer

Harry Thomas

Makeup Artist

Scott McCloud

Second Unit

Ben Winkler

Sound Recordist

Bert Shipman

Camera Operator

Larry Smith

Still Photographer

Jack Miles

Settings

Sanford H. Dickinson

Music Supervisor

Bud Schelling

Editor

Nathan Bailey

Medical Consultant